Measuring Protein Synthesis With SUnSET

Measuring mitochondrial protein synthesis to assess biogenesis.

IN THEIR PERSPECTIVES ARTICLE, Miller and Hamilton (6) propose that the term “mitochondrial biogenesis” be limited to the measurement of the synthesis rate of mitochondrial proteins. For support, they cite evidence that changes in the abundance of transcription factors for mitochondrial genes or the mRNAs encoding mitochondrial proteins may not result in corresponding changes in mtDNA or protei...

Measuring protein synthesis by mass isotopomer distribution analysis (MIDA).

The measurement of protein kinetics by isotopic techniques has been hindered by the long-standing difficulty of accurately measuring the isotope content of the biosynthetic precursor pool (aminoacyl-tRNA in tissues). Mass isotopomer distribution analysis (MIDA) is a stable isotope-mass spectrometric (MS) technique for measuring biosynthetic precursor enrichments from measurements on a polymeric...

Measuring vocal quality with speech synthesis.

Much previous research has demonstrated that listeners do not agree well when using traditional rating scales to measure pathological voice quality. Although these findings may indicate that listeners are inherently unable to agree in their perception of such complex auditory stimuli, another explanation implicates the particular measurement method-rating scale judgments-as the culprit. An alte...

An Eclipse before Sunset

Mountain Village at Sunset.

During my travels to numerous countries over 40 years, I have seen many picturesque mountain villages with elegant and well-preserved houses perched on carefully selected sites on the mountains, and others located by a river or lake at the foot of a mountain. They appear remarkably harmonious with the natural scenery. Fruit trees or tea trees and crops such as rice, wheat, and corn are planted ...